1.?磋???17c?戒??锋按????????绁?绉?瀵???锛?褰????剁???藉????涓?灏?绔????

?ㄦ旦???????界?瀛﹂???锛??绘??涓?浜??拌薄锛????剁???规?у??娣遍??????娑碉?寮??????????????充??㈢储??浠?澶╋???浠?????????︿?涓?涓????峰???绘?т?浠や汉?????姒?蹇碘??????17c?戒??锋按????????杩?涓???浼煎??婊¤?????琛ㄨ堪锛?瀹????村????涓??烘??ㄦ???跺??????绉????╁?斤?瀹?????????浠?????戒慨澶?涓???????浼?缁?璁ょ?ワ?涓轰汉绫诲?ュ悍?????借川??????????寮?浜??ㄦ?扮??缁村害??

??17c??????杩?涓??板?缁???锛?骞堕??绠???????ヤ唬??锛?瀹?浠ｐ???〃??涓?涓??瑰??????╁??瀛?搴???锛??????绉???杩??????╁伐绋?????钩?般???ㄧ???浜哄????涓????㈢储涓??绉?瀛﹀?浠???濂??板???帮??ㄧ?瑰??′欢涓?锛???浜????╀?锛???瀹?楠?瀹ゆ??寤虹?????╂ā??锛?灞??板?轰????????????????藉??锛??舵?稿??哄?朵???17c??搴?????????揣瀵??稿?炽??

?????戒??锋按???????绉?褰㈣薄??姣?????伙??ㄤ互??缁?杩?绉?????杩?绋???绮惧?涓?楂?????瀹????芥??浠ｇ????????ㄤ慨澶?杩?绋?涓??浠ヤ?绉???搴???娴?????涓??锋?????芥椿?????舵??锛?濡???缁?瀵???涓?绾胯??簿??瀵规?ワ?骞堕???惧?哄?抽???????╂椿?х?╄川锛?浠???瀹??版??浼ょ??蹇???寮ュ??涓????界???㈠???杩?绉????锋??????虹?????藉??锛???????ㄥ己澶у??椹卞??涓?澶??ㄥ??杩???????????椹卞?ㄤ?锛?杩稿???虹????浜烘??姐??

?挎??浠ユ?ワ?浜虹被瀵逛???浼ゅ??琛拌?????垫??锛?涓昏?渚?璧?浜?澶??ㄧ???荤??骞查?锛?濡??????????╂不??绛?????17c?戒??锋按?????????虹?帮???????浜?涓?绉??翠负?规???х??瑙ｅ?虫?规?????婵?娲诲??澧?寮虹???╀???韩??淇???藉????杩?灏卞???????′?涓????藉?????淇??????涓??介?ュ????锛??藉?瑙ｉ??缁???娣卞???娼??斤?浣垮?跺?ㄩ?㈠???浼ゆ?讹?涓??????????ㄧ?寰?澶???????锛?????富?ㄥ???ㄤ??虹簿瀵???????????????宸ョ???

??浠??濡ㄨ??充?涓?锛?褰?韬??????涓??ㄤ???????浼わ?渚?濡?缁?缁???瑁????ㄥ?琛扮?锛????????剧??瀵拣????寸??缁???姝讳骸锛?浼?缁???娌荤??寰?寰?????淇?ˉ???????挎?⑩????浣???17c?戒??锋按????????浠ｈ〃??????????村??浜?????濉???????????????瀹????介??杩?寮??ユ??婵?娲荤?瑰????哄????璺??寮?瀵肩???杩?琛?瀹???????锛?浠ヤ?绉?楂?搴︾簿?????瑰?锛?妯℃?????????叉?舵????绁?濂?杩?绋?锛?浠????ㄦ??浼ら?ㄤ??????库???哄?ㄦ?扮???????藉??寸??缁?缁????ㄥ???

杩?绉??????藉??锛?骞堕??绉?骞诲?璇翠腑??????锛??????轰?瀵圭???芥??川娣卞?荤??瑙ｅ????娌跨???╃???????????

???戒?????姣??伙?灏ゅ?跺?浜哄?ヨ????瀹???绀轰??ㄨ????杩?绋?涓??淇℃????浼??????╄川??杈???浠ュ??缁????寸????ュ??璋?锛??藉???绮惧???涓?缃???氦缁???渚?濡?锛???杩???绾崇背?哄?ㄤ汉?????╀?????????藉?ㄥ井瑙?灞??㈢??娴???浼ゆ???碉?骞剁簿纭??板?淇????瀛??????夸俊?锋??骞茬???寮?瀵艰?虫????瑕????版?广??杩?浜?绾崇背绾х????涓?绾库??锛?灏?淇℃?????╄川浠ヨ?涔?瀹?缇???????浼???锛?纭??姣?涓?娆○??淇???芥???娆＄簿?????????扳??????缂?缁?????

?????锋按?????磋?涓?姝ワ???缁?浜?淇??杩?绋????ㄦ???у?????藉????瀹????芥???崇???ㄤ慨澶?杩?绋?涓????澶ч?????介?????ュ?荤?╄川???抽??俊?峰??瀛?琚?????扳??杈????????????锯??锛?涓虹?????蹇???澧?娈???杩?绉诲????????渚???瓒崇???ㄥ??锛?浠???瀹??扳????浼ょ????杩硅???琚????藉??????ユ椽娴???娣规病??????薄??

褰?????讹???17c?戒??锋按??????骞堕??榄?娉?锛??????瀛︾??缁??躲???惰?????????芥???澶?椤归?瑕??ф????????ュ????浣?????????涓??????浜?锛?

绮惧???哄??缂?杈??????濡?CRISPR-Cas9????绾х??锛?锛??藉?绮剧‘淇??规??婵?娲讳??????藉???稿?崇???哄??锛?浠???璧?浜?缁????村己??淇??娼??姐????杩???骞茬?????????╃?ㄨ?瀵煎??藉共缁???锛?iPSCs锛?????浣?骞茬???锛??ㄤ?澶??瑰?诲苟璇卞??跺????涓虹?瑰?绫诲????缁???锛???灏??剁Щ妞??版??浼ら?ㄤ?锛?瀹??扮?缁???????

绾崇背???╂??????????藉??ㄤ???绮剧‘?跺???????捐???╂???ц?淇??浠诲?＄??绾崇背杞戒???绾崇背?哄?ㄤ汉锛?瀹?浠???互??褰?寰??????寤虹?宸モ??锛?寮?瀵煎??杈??╄????杩?绋??????╀俊?疯??э?娣卞?ョ??瑙ｅ??妯℃?????戒?????淇″?烽??璺????杩?澶?婧??т俊?峰??瀛???绮剧‘缁?浜?锛?璋??х???琛?涓猴?淇?????淇??????????

??17c?戒??锋按??????姒?蹇电?????猴???蹇?????浠???ㄤ???瀵规???剧????杞??????ユ?卞?ュ悍??锛?浠???寤堕?垮??解??杞????????????借川??????瀹?灏???濉???浠??琛拌?????剧??????浼ょ????瑙ｏ?骞跺???戒负璁稿???????娉?娌绘?????剧????渚??╁?芥?х??瑙ｅ?虫?规???杩?涓?浠?浠????娆℃???????绐??达??存???娆″????藉?插???浜虹被???ュ?借???娣卞?诲??开??

?ヤ??ョ???ㄥ??锛???浠??娣卞?ユ?㈣?杩?椤圭??????藉甫?ョ???蜂?搴????浠ュ??瀹?灏?濡?浣??瑰????浠???涓?????

2.绉???涔???锛???濉????斤?17c?戒??锋按?????????ュ?炬???????????/p>

?ㄥ??涓??ㄥ??锛???浠???姝ユ???浜???17c?戒??锋按??????杩?涓?姒?蹇电????ョ?绉??㈢罕锛?灏??朵?灏?绔??????╃????????戒慨澶??????ョ揣瀵???绯汇??濡?浠?锛?璁╂??浠???????筹?褰?杩?椤归?瑕??х????????姝ｇ?ц??板?锛?瀹?灏?濡?浣?浠モ???戒??锋按?????????藉??锛??ㄥ??涓????缁??跺?轰?骞?骞?浠や汉???圭?????ュ?炬????

?ㄧ?剧??娌荤??棰???锛???17c?戒??锋按??????灏?甯︽?ュ????????????????瀵逛??ｄ???缁?????浼ゆ?????介??????瀵艰?寸???剧??锛?濡?蹇?????锛?蹇???姊?姝诲??蹇???淇??锛???绁?缁???琛??х?剧??锛?濡?甯???妫????筐???灏??ㄦ捣榛???涓??缁?????????锛??????????捐?????㈡?ф??浼わ?杩?椤规??????????渚????规???р????瑙ｅ?虫?规???

?宠薄涓?涓?锛???杩?涓?娆＄簿?????哄??璋??ф??缁???绉绘?锛???????蹇????藉????扮???????猴???????绁?缁????藉????拌??ワ?琛扮????ㄥ??藉??峰??扮????杩?涓????????????????剁?瑙ｏ???????姝ｆ??涔?涓?????モ????杞?“????淇????浼も????

渚?濡?锛??ㄥ?琛?绠＄?剧??????娌荤??涓??浼?缁??????舵??ユ????ˉ?????浠?浠????╃??涓?????????????????17c?戒??锋按????????????????介??杩?婵?娲诲???缁??????????藉??锛?????瀹????瑰?诲??绉绘??扮??蹇???缁???锛?璁┾??姝讳骸??????ュ??????扳??璺冲?ㄢ??璧锋?ャ??杩???????????介〗?哄?″????涓?娆￠??澶х??淬??

?ㄦ??浼や慨澶?涓???浼ょ????归????跺??ㄥ???峰????箍??????璁烘????澶?浜?????????涓ラ????浼わ?濡??т激???????杩????????澶?绉??????浼?缁???搴峰?杩?绋?婕??夸?????????????17c?金??′??锋按???????????????澶уぇ缂╃???????堕?达???楂?淇??璐ㄩ??锛????冲??板???界??楂?搴︽?㈠????宠薄涓?涓?锛?涓ラ?????т激?ｈ??锛??剁???よ?藉?浠ユ?村揩????搴︺???村????ょ??杩?琛?????锛????㈢?????锛??ㄧ??璁轰????介??杩???????????㈠??堕?ㄥ?????ㄩ?ㄥ???姐??

杩?绉???蹇?????楂?????楂?淇???????淇???藉??锛?灏???澶у?板??杞绘?ｈ??????ョ???︼???????娲昏川????

灏ゅ?跺?煎?涓?????????ㄦ??琛拌??涓?寤剁?琛拌??棰???锛???17c?戒??锋按??????灞??板?轰?宸ㄥぇ??娼?????琛拌???????戒????惰???剁??杩?绋?锛??舵??川?????????浼ょ疮绉??????借“?????存?拌?藉??涓?????濡????藉???杩???17c?????????缁??娲绘??澧?寮鸿韩浣???????淇?????????藉??锛??ｄ???琛拌??????杩?绋????﹀??互琚???????寤剁?锛????冲?ㄤ?瀹?绋?搴︿?琚?????杞???锛?杩?骞堕?????崇??姘哥??锛???????锛?涓???藉?浠ユ?村?ュ悍???村勾杞荤???舵??锛??ユ???撮?跨???ュ悍瀵垮?斤?瀹??扳???ュ悍??榫???????

杩?瀵逛?瑙ｅ?冲?ㄧ????榫???甯︽?ョ??绀句???缁?娴?????锛??锋?????朵唬????涔???

?ㄥ?????诲?涓??ㄥ?绉绘?棰???锛???17c?戒??锋按?????????藉交搴??瑰?????????煎????ㄥ???己涓??存???版?扮Щ妞??诲????鹃?????濡????藉??╃?ㄨ?椤规??????ㄤ?澶??????扳???????硅?测???哄???藉??寸???ㄥ?锛??????存?ュ?ㄤ???璇卞??ㄥ???????锛??ｄ??ㄥ?绉绘????堕?灏?涓?澶?瀛??ㄣ??杩?涓?浠??藉??芥???村????斤?杩??芥??ゅ??渚?浣????ュ??搴????挎?????╂???剁?甯︽?ョ??椋??╋?璁┾???ㄨ??繁??缁????硅?茶??繁???ㄥ?????涓虹?板???

褰??讹???浠????瑕?璁よ??帮???17c?戒??锋按????????瀹??帮?骞堕??涓?韫磋??灏便??瀹???瑕?璺ㄥ?绉???绱у???浣?锛???瑕?娴烽???????????ワ??撮??瑕?绐??翠鸡????娉?寰???绀句??ュ??搴︾??澶?????????渚?濡?锛??哄??缂?杈???????瀹??ㄦ?с??骞茬??????挎??绋冲??с??绾崇背?哄?ㄤ汉??浣???瀹??ㄦ?х?锛??介??瑕?缁?杩?涓ユ?肩??绉?瀛﹂?璇???瀹℃????璇?浼般??

浣???璁哄?浣?锛?杩?涓??蹇垫??浠ｈ〃???瑰??锛???????汉绫诲????芥?㈢储????涓?娆′?澶ч?璺?????17c?戒??锋按??????锛?瀹?涓?浠????涓??浜哄?ヨ????绉?瀛︾???筹??存????浠??涓?涓??村?ュ悍???撮?垮????存??娲诲???????ョ??缇?濂芥???笺??瀹?灏卞???????????涓???????锛?棰?绀虹??绉???涔???灏?褰诲??逛寒???戒慨澶?涓??????????????斤?涓轰汉绫绘??????杩?姝ユ敞?ュ??????????寮哄ぇ?ㄥ????

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